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anti p smad1 5  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti p smad1 5
    Anti P Smad1 5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+smad1+5/pmc13051983-57-12-14
    Average 86 stars, based on 1 article reviews
    anti p smad1 5 - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    other:

    Article Title: Positional BMP signaling orchestrates villus length in the small intestine
    Article Snippet: Mice injected with adenovirus expressing R-spondin1 were sacrificed 5 days later.

    Article Title: Positional BMP signaling orchestrates villus length in the small intestine.
    Article Snippet: One milligram/mL poly(I:C) HMW (InvivoGen, tlrl-pic) was made, and 20mg/kg was injected.

    Incubation:

    Article Title: OSMR coordinates a self-perpetuating circuit linking chemoresistance and neutrophil-driven immunosuppression in gastric cancer
    Article Snippet: Equal amounts of protein (20–50 μg) were separated by 10% SDS-PAGE and transferred onto NC membranes using a semi-dry transfer system (Bio-Rad, USA). .. The membranes were blocked with 5% non-fat milk in TBST for 1 hour at room temperature, followed by an overnight incubation at 4°C with primary antibodies (OSMR (Abcam, #ab315388), CCNE2 (Abcam, #ab40890), β-actin (CST, #4967), PI3K (P85) (CST, #4292), p-PI3K (p-P85) (Abcam, #ab278545), AKT (CST, #9272), p-AKT (CST, #4060), P27 (CST, #2552), p-P27 (Abcam, #ab62364), FLAG (CST, #14793), BMP5 (Proteintech, #13253-1-AP), Ub (CST, #20326), TRIM2 (Proteintech, #20356-1-AP), GATA3 (CST, #5852), p-SMAD1/5 (CST, #9516), SMAD5 (CST, #12534), PD-L1 (CST, #13684), BATF (CST, #8638), IL-31 (abcam, #ab62579)), which were diluted in accordance with the manufacturer's instructions. ..

    Article Title: Paeonol alleviates pulmonary arterial hypertension by activation of BRCC3
    Article Snippet: Background: Pulmonary arterial hypertension (PAH) is characterized by vascular remodeling and right ventricular failure.. Paeonol, a bioactive compound extracted from the root bark of Paeonia suffruticosa, exhibits antiinflammatory, antioxidant, and anticancer effects.. However, its efficacy on pulmonary vasculature in the context of PAH remains unknown.

    Article Title: OSMR coordinates a self-perpetuating circuit linking chemoresistance and neutrophil-driven immunosuppression in gastric cancer.
    Article Snippet: Equal amounts of protein (20–50 μg) were separated by 10% SDS-PAGE and transferred onto NC membranes using a semi-dry transfer system (Bio-Rad, USA). .. The membranes were blocked with 5% non-fat milk in TBST for 1 hour at room temperature, followed by an overnight incubation at 4◦C with primary antibodies (OSMR (Abcam, #ab315388), CCNE2 (Abcam, #ab40890), β-actin (CST, #4967), PI3K (P85) (CST, #4292), p-PI3K (p-P85) (Abcam, #ab278545), AKT (CST, #9272), p-AKT (CST, #4060), P27 (CST, #2552), p-P27 (Abcam, #ab62364), FLAG (CST, #14793), BMP5 (Proteintech, #13253-1-AP), Ub (CST, #20326), TRIM2 (Proteintech, #20356-1-AP), GATA3 (CST, #5852), p-SMAD1/5 (CST, #9516), SMAD5 (CST, #12534), PD-L1 (CST, #13684), BATF (CST, #8638), IL-31 (abcam, #ab62579)), which were diluted in accordance with the manufacturer's instructions. ..

    Western Blot:

    Article Title: One-step strategy for fabricating icariin-encapsulated biomimetic Scaffold: Orchestrating immune, angiogenic, and osteogenic cascade for enhanced bone regeneration
    Article Snippet: On day 7, ALP staining and quantitative analysis as well as the IF staining of RUNX2 (ab236639, Abcam, UK), BMP2 (ab284387, Abcam, UK), and OCN (ab93876, Abcam, UK) were performed. .. Moreover, in order to study the regulatory pathway of macrophage supernatant on osteogenic differentiation behavior of BMSCs, the relative protein levels of BMP2 (ab284387, Abcam, UK), OCN (ab93876, Abcam, UK), p-SMAD1/5 (9516, cell signaling technology, USA), β-Actin (ab6728, Abcam, UK), and GAPDH (ab8245, Abcam, UK) in BMSCs were measured via WB assay. ..

    Cell-Signaling:

    Article Title: Regulating the cell differentiation trajectory of progenitor cells in adipose tissue fibrosis.
    Article Snippet: .. The following primary antibodies from Cell Signaling Technology (CST) were diluted 1:1,000: peroxisome proliferator activated receptor gamma (PPARγ), Perilipin-1, hormonesensitive lipase (HSL), phospho(p)-HSL, TGFβ1, SMAD2/3, p-SMAD3, p-SMAD1/5, and SMAD4. .. Primary antibodies against glyceraldehyde3-phosphate dehydrogenase (GAPDH) from CST were diluted 1:5,000.

    Blocking Assay:

    Article Title: Paeonol alleviates pulmonary arterial hypertension by activation of BRCC3
    Article Snippet: Background: Pulmonary arterial hypertension (PAH) is characterized by vascular remodeling and right ventricular failure.. Paeonol, a bioactive compound extracted from the root bark of Paeonia suffruticosa, exhibits antiinflammatory, antioxidant, and anticancer effects.. However, its efficacy on pulmonary vasculature in the context of PAH remains unknown.

    Saline:

    Article Title: Paeonol alleviates pulmonary arterial hypertension by activation of BRCC3
    Article Snippet: Background: Pulmonary arterial hypertension (PAH) is characterized by vascular remodeling and right ventricular failure.. Paeonol, a bioactive compound extracted from the root bark of Paeonia suffruticosa, exhibits antiinflammatory, antioxidant, and anticancer effects.. However, its efficacy on pulmonary vasculature in the context of PAH remains unknown.



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    Cytokines Activated Their Downstream Pathways in HK-2 Cells. The phosphorylation of ERK, JNK, p38, and NF-κB in HK-2 cells treated with TNF-α (A) or IL-1β (B) was analyzed over a time course ranging from 15 min to 4 h. The <t>phosphorylation</t> <t>of</t> <t>Smad1/5/9</t> and Smad2/3 in HK-2 cells treated with TGF-β1 (C) or BMP-2 (D) was analyzed over a time course ranging from 15 min to 4 h. GAPDH was served as the loading control.
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    Cytokines Activated Their Downstream Pathways in HK-2 Cells. The phosphorylation of ERK, JNK, p38, and NF-κB in HK-2 cells treated with TNF-α (A) or IL-1β (B) was analyzed over a time course ranging from 15 min to 4 h. The <t>phosphorylation</t> <t>of</t> <t>Smad1/5/9</t> and Smad2/3 in HK-2 cells treated with TGF-β1 (C) or BMP-2 (D) was analyzed over a time course ranging from 15 min to 4 h. GAPDH was served as the loading control.
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    Cytokines Activated Their Downstream Pathways in HK-2 Cells. The phosphorylation of ERK, JNK, p38, and NF-κB in HK-2 cells treated with TNF-α (A) or IL-1β (B) was analyzed over a time course ranging from 15 min to 4 h. The <t>phosphorylation</t> <t>of</t> <t>Smad1/5/9</t> and Smad2/3 in HK-2 cells treated with TGF-β1 (C) or BMP-2 (D) was analyzed over a time course ranging from 15 min to 4 h. GAPDH was served as the loading control.
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    Tumor BMP5 activates <t>the</t> <t>SMAD1/5</t> pathway in neutrophils and promotes PD-L1 transcription. (A) Correlation between neutrophil score and BMP5 receptors in TCGA database. (B) Schematic of BMP5 and its receptors signaling pathway. (C) p-SMAD1/5 and SMAD protein levels in neutrophils. (D) PD-L1 mRNA and protein level in neutrophils treated with BMP5 and SMAD1/5 inhibitor. (E) PD-L1 mRNA and protein level in neutrophils co-cultured with MKN28 cells or treated with SMAD1/5 inhibitor. (F) Dual-Luciferase Reporter Assay analysis for SMAD5-binding PD-L1 promoter. (G) mIF staining and correlation of BMP5,CD15, PD-L1, and CD8 in tumor samples from patients in Cohort 2. (**, P < 0.01).
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    Tumor BMP5 activates <t>the</t> <t>SMAD1/5</t> pathway in neutrophils and promotes PD-L1 transcription. (A) Correlation between neutrophil score and BMP5 receptors in TCGA database. (B) Schematic of BMP5 and its receptors signaling pathway. (C) p-SMAD1/5 and SMAD protein levels in neutrophils. (D) PD-L1 mRNA and protein level in neutrophils treated with BMP5 and SMAD1/5 inhibitor. (E) PD-L1 mRNA and protein level in neutrophils co-cultured with MKN28 cells or treated with SMAD1/5 inhibitor. (F) Dual-Luciferase Reporter Assay analysis for SMAD5-binding PD-L1 promoter. (G) mIF staining and correlation of BMP5,CD15, PD-L1, and CD8 in tumor samples from patients in Cohort 2. (**, P < 0.01).
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    Tumor BMP5 activates <t>the</t> <t>SMAD1/5</t> pathway in neutrophils and promotes PD-L1 transcription. (A) Correlation between neutrophil score and BMP5 receptors in TCGA database. (B) Schematic of BMP5 and its receptors signaling pathway. (C) p-SMAD1/5 and SMAD protein levels in neutrophils. (D) PD-L1 mRNA and protein level in neutrophils treated with BMP5 and SMAD1/5 inhibitor. (E) PD-L1 mRNA and protein level in neutrophils co-cultured with MKN28 cells or treated with SMAD1/5 inhibitor. (F) Dual-Luciferase Reporter Assay analysis for SMAD5-binding PD-L1 promoter. (G) mIF staining and correlation of BMP5,CD15, PD-L1, and CD8 in tumor samples from patients in Cohort 2. (**, P < 0.01).
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    Image Search Results


    Cytokines Activated Their Downstream Pathways in HK-2 Cells. The phosphorylation of ERK, JNK, p38, and NF-κB in HK-2 cells treated with TNF-α (A) or IL-1β (B) was analyzed over a time course ranging from 15 min to 4 h. The phosphorylation of Smad1/5/9 and Smad2/3 in HK-2 cells treated with TGF-β1 (C) or BMP-2 (D) was analyzed over a time course ranging from 15 min to 4 h. GAPDH was served as the loading control.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Inflammation-induced osteogenic signaling promotes calcium phosphate crystal formation in kidneys via MAPK, NF-κB, and smad pathways

    doi: 10.3389/fcell.2026.1831072

    Figure Lengend Snippet: Cytokines Activated Their Downstream Pathways in HK-2 Cells. The phosphorylation of ERK, JNK, p38, and NF-κB in HK-2 cells treated with TNF-α (A) or IL-1β (B) was analyzed over a time course ranging from 15 min to 4 h. The phosphorylation of Smad1/5/9 and Smad2/3 in HK-2 cells treated with TGF-β1 (C) or BMP-2 (D) was analyzed over a time course ranging from 15 min to 4 h. GAPDH was served as the loading control.

    Article Snippet: Primary antibodies for immunoblotting such as OPN (A19092), ALP (A0514), OPG (A2100), Runx2 (A2851), p-JNK1/2/3 (AP0631), JNK1/2/3 (A4867), p-NF-κB (AP0944), NF-κB (A19653), p-p38 (AP0057), p38 (A14401), p-ERK (AP0485), ERK (A4782), p-Smad1/5/9 (AP0850), Smad1 (A21734), p-Smad2/3 (AP0548), Smad2/3 (A7536), TNF-α (A11534), IL-1β (A11534), TGF-β1 (A16640), BMP-2 (A0231), β-actin (AC028), α-Tubulin (A6830), and GAPDH (A19056) were from ABclonal (Woburn, MA, United States).

    Techniques: Phospho-proteomics, Control

    Tumor BMP5 activates the SMAD1/5 pathway in neutrophils and promotes PD-L1 transcription. (A) Correlation between neutrophil score and BMP5 receptors in TCGA database. (B) Schematic of BMP5 and its receptors signaling pathway. (C) p-SMAD1/5 and SMAD protein levels in neutrophils. (D) PD-L1 mRNA and protein level in neutrophils treated with BMP5 and SMAD1/5 inhibitor. (E) PD-L1 mRNA and protein level in neutrophils co-cultured with MKN28 cells or treated with SMAD1/5 inhibitor. (F) Dual-Luciferase Reporter Assay analysis for SMAD5-binding PD-L1 promoter. (G) mIF staining and correlation of BMP5,CD15, PD-L1, and CD8 in tumor samples from patients in Cohort 2. (**, P < 0.01).

    Journal: Neoplasia (New York, N.Y.)

    Article Title: OSMR coordinates a self-perpetuating circuit linking chemoresistance and neutrophil-driven immunosuppression in gastric cancer

    doi: 10.1016/j.neo.2026.101279

    Figure Lengend Snippet: Tumor BMP5 activates the SMAD1/5 pathway in neutrophils and promotes PD-L1 transcription. (A) Correlation between neutrophil score and BMP5 receptors in TCGA database. (B) Schematic of BMP5 and its receptors signaling pathway. (C) p-SMAD1/5 and SMAD protein levels in neutrophils. (D) PD-L1 mRNA and protein level in neutrophils treated with BMP5 and SMAD1/5 inhibitor. (E) PD-L1 mRNA and protein level in neutrophils co-cultured with MKN28 cells or treated with SMAD1/5 inhibitor. (F) Dual-Luciferase Reporter Assay analysis for SMAD5-binding PD-L1 promoter. (G) mIF staining and correlation of BMP5,CD15, PD-L1, and CD8 in tumor samples from patients in Cohort 2. (**, P < 0.01).

    Article Snippet: The membranes were blocked with 5% non-fat milk in TBST for 1 hour at room temperature, followed by an overnight incubation at 4°C with primary antibodies (OSMR (Abcam, #ab315388), CCNE2 (Abcam, #ab40890), β-actin (CST, #4967), PI3K (P85) (CST, #4292), p-PI3K (p-P85) (Abcam, #ab278545), AKT (CST, #9272), p-AKT (CST, #4060), P27 (CST, #2552), p-P27 (Abcam, #ab62364), FLAG (CST, #14793), BMP5 (Proteintech, #13253-1-AP), Ub (CST, #20326), TRIM2 (Proteintech, #20356-1-AP), GATA3 (CST, #5852), p-SMAD1/5 (CST, #9516), SMAD5 (CST, #12534), PD-L1 (CST, #13684), BATF (CST, #8638), IL-31 (abcam, #ab62579)), which were diluted in accordance with the manufacturer's instructions.

    Techniques: Cell Culture, Luciferase, Reporter Assay, Binding Assay, Staining